Rhodus N L, Liljemark W, Bloomquist C, Bereuter J
Division of Oral Medicine-Diagnosis, University of Minnesota, School of Dentistry, Minneapolis, USA.
Quintessence Int. 1998 Nov;29(11):705-10.
The purpose of this study was to compare the quantities of oral Candida albicans in patients with primary and secondary Sjögren's syndrome before and after the use of orally administered pilocarpine hydrochloride for 1 year.
Twelve female subjects with primary (n = 4) and secondary (n = 8) Sjögren's syndrome (mean age +/- SEM = 56.7 +/- 5.7 years) were enrolled in the study, after meeting rigid enrollment criteria. Oropharyngeal collection of samples and culturing was performed on each subject. Cultures specific for Candida albicans were plated into a culture media tube using the Oricult kit and also by serial dilutions and plating by a streptomycin-vancomycin technique. Cultures were incubated for 48 hours at 37 degrees C. The subjects used 5 mg of pilocarpine hydrochloride, administered orally three times daily, for 1 year, after which both of the Candida cultures were repeated. None of the subjects used antifungal medications, none smoked, and all were dentate.
There was a significant difference in the prevalence of Candida after the use of pilocarpine hydrochloride for both groups. At the start of the study, 75% of all subjects were positive for Candida. Following the use of pilocarpine, 25% had positive cultures. There was also a decrease in the prevalence of clinical manifestations of infection from 75% of subjects to 25%. There was a significant decrease in the numbers of Candida cultured following the use of pilocarpine.
Long-term administration of pilocarpine hydrochloride resulted in a significant reduction in Candida albicans colonization in patients with primary or secondary Sjögren's syndrome.
本研究旨在比较原发性和继发性干燥综合征患者口服盐酸毛果芸香碱1年前后口腔白色念珠菌的数量。
12名患有原发性（n = 4）和继发性（n = 8）干燥综合征的女性受试者（平均年龄±标准误= 56.7±5.7岁）在符合严格的入选标准后被纳入研究。对每个受试者进行口咽样本采集和培养。使用Oricult试剂盒并通过链霉素-万古霉素技术进行系列稀释和平板接种，将白色念珠菌特异性培养物接种到培养基管中。培养物在37℃下孵育48小时。受试者口服5毫克盐酸毛果芸香碱，每日3次，持续1年，之后重复进行两种念珠菌培养。所有受试者均未使用抗真菌药物，均不吸烟，且均有牙齿。
两组在使用盐酸毛果芸香碱后念珠菌的患病率有显著差异。在研究开始时，所有受试者中有75%念珠菌呈阳性。使用毛果芸香碱后，25%培养物呈阳性。感染临床表现的患病率也从75%的受试者降至25%。使用毛果芸香碱后培养出的念珠菌数量显著减少。
长期服用盐酸毛果芸香碱可使原发性或继发性干燥综合征患者白色念珠菌定植显著减少。
