Rhodus N L, Bloomquist C, Liljemark W, Bereuter J
Division of Oral Medicine, School of Dentistry, University of Minnesota, Minneapolis, USA.
Quintessence Int. 1998 Feb;29(2):107-13.
An immediate chairside technique (Latex Candida) for the detection of Candida albicans was compared with a simple tube culturing technique (Oricult) and the traditional laboratory culturing technique in patients with Sjögren's syndrome.
Subjects with primary (n = 9) and secondary (n = 9) Sjögren's syndrome (mean age of 56.7 years; all female) and an age- and sex-matched group of control subjects (n = 9) were selected. Three different methods for culturing Candida albicans were performed for each subject. One culturette was plated on a trypticase soy-agar streptomycin-vancomycin medium plate and incubated for 48 hours at 37 degrees C. Another swab was plated on a reagent paper with the Latex Candida test kit. The third swab was placed in a culture media tube using the Oricult kit and incubated for 48 hours at 37 degrees C.
All three techniques indicated a significant difference in the prevalence of Candida between the control group and both Sjögren's groups. The Latex Candida technique indicated that 78% of all Sjögren's subjects were positive for Candida, while the other two tests indicated that 83% were positive.
The Latex Candida technique was comparable to Oricult and streptomycin-vancomycin culturing techniques for negative results and was correctly positive for 90% of cases.
在干燥综合征患者中，将用于检测白色念珠菌的即时椅旁技术（乳胶念珠菌法）与简单的试管培养技术（Oricult法）及传统实验室培养技术进行比较。
选取原发性干燥综合征患者9例、继发性干燥综合征患者9例（平均年龄56.7岁；均为女性），以及年龄和性别匹配的对照组9例。对每位受试者采用三种不同的白色念珠菌培养方法。将一个培养管接种于胰蛋白酶大豆琼脂链霉素 - 万古霉素培养基平板上，于37℃孵育48小时。另一拭子用乳胶念珠菌检测试剂盒接种于试剂纸上。第三个拭子使用Oricult试剂盒置于培养基管中，于37℃孵育48小时。
所有三种技术均显示对照组与两个干燥综合征组之间念珠菌感染率存在显著差异。乳胶念珠菌技术显示所有干燥综合征受试者中有78%念珠菌检测呈阳性，而其他两项检测显示阳性率为83%。
乳胶念珠菌技术在阴性结果方面与Oricult法及链霉素 - 万古霉素培养技术相当，且在90%的病例中检测结果正确呈阳性。
