Friel J K, Andrews W L, Jackson S E, Longerich H P, Mercer C, McDonald A, Dawson B, Sutradhar B
Department of Biochemistry, Memorial University of Newfoundland, St. John's, Canada.
Biol Trace Elem Res. 1999 Mar;67(3):225-47. doi: 10.1007/BF02784423.
To examine longitudinal and gestational effects of mineral content in human milk, we analyzed human milk from lactating mothers of premature (PRT, n = 24, < 2000 g birth weight, < 37 wk gestation) and full-term (FT, n = 19, > 2500 g, 39-41 wk gestation), living in Newfoundland, Canada. Samples were collected once a week for 8 wk with one final sample collected at 3 mo. Milk samples collected in acid-washed containers were wet ashed with concentrated HNO3, and barium, cadmium, calcium, cesium, cobalt, copper, cerium, lanthanum, magnesium, manganese, molybdenum, nickel, lead, rubidium, tin, strontium, and zinc were measured using inductively coupled plasma-mass spectrometry. Data were analyzed using standard multiple-regression procedures with correlated data analyses to take account of the relationship between successive weeks. Results indicated lower Ca and Pb in PRT milk. Calcium was the only nutritionally significant element to differ between groups. Molybdenum in both PRT and FT milk showed a definite decrease with time, suggesting that the Mo content in milk is homeostatically regulated. However, Ce, La, Ba, and Sn did not display any pattern indicative of biological regulation and potential human requirement.
为研究母乳中矿物质含量的纵向和孕期影响，我们分析了加拿大纽芬兰地区早产（PRT，n = 24，出生体重<2000 g，孕期<37周）和足月产（FT，n = 19，>2500 g，孕期39 - 41周）哺乳期母亲的母乳。样本每周采集一次，共采集8周，最后在3个月时采集一个样本。采集于酸洗容器中的母乳样本用浓硝酸进行湿消化，然后使用电感耦合等离子体质谱法测量钡、镉、钙、铯、钴、铜、铈、镧、镁、锰、钼、镍、铅、铷、锡、锶和锌的含量。数据分析采用标准多元回归程序及相关数据分析，以考虑连续几周之间的关系。结果表明，早产母乳中的钙和铅含量较低。钙是两组之间唯一在营养上有显著差异的元素。早产母乳和足月母乳中的钼含量均随时间呈明显下降趋势，这表明母乳中的钼含量受到稳态调节。然而，铈、镧、钡和锡并未呈现出任何表明生物调节和潜在人体需求的模式。
