Mukasa A, Born W K, O'Brien R L
National Jewish Medical and Research Center, Denver, CO 80206, USA.
J Immunol. 1999 Apr 15;162(8):4910-3.
Whether gamma delta T lymphocytes respond to microbial Ags or to inducible host Ags remains a matter of controversy. Using several different disease models and mouse strains, we and others have seen that V gamma 6/V delta 1 gamma delta T cells preferentially increase among the gamma delta T cells infiltrating inflamed tissues. However, it was not clear whether bacteria are necessary to bring about this response. Therefore, we have reexamined this question using a disease model in which inflammation is induced by a purely autoimmune process involving no bacteria, bacterial products, or other foreign material: testicular cell-induced autoimmune orchitis. Using this model we found that gamma delta T cells were still plentiful among the infiltrating T lymphocytes, being 9- to 10-fold more prevalent than in spleen, and that V gamma 6/V delta 1+ cells again represented the predominant gamma delta T cell type. This finding shows that the response of the V gamma 6/V delta 1+ subset does not, in fact, depend upon the presence of bacteria or bacterial products. The stimulus triggering the response of the V gamma 6/V delta 1 gamma delta T cells appears to be neither foreign nor organ-specific in origin, but instead consists of a self-derived host Ag or signal induced during the inflammatory process.
γδ T淋巴细胞是对微生物抗原还是对诱导性宿主抗原产生应答，仍是一个有争议的问题。利用几种不同的疾病模型和小鼠品系，我们和其他人已经观察到，在浸润炎症组织的γδ T细胞中，Vγ6/Vδ1 γδ T细胞优先增加。然而，尚不清楚细菌对于引发这种反应是否必要。因此，我们使用一种疾病模型重新审视了这个问题，在该模型中，炎症是由不涉及细菌、细菌产物或其他异物的纯自身免疫过程诱导的：睾丸细胞诱导的自身免疫性睾丸炎。使用这个模型，我们发现γδ T细胞在浸润的T淋巴细胞中仍然大量存在，其数量比脾脏中的多9至10倍，并且Vγ6/Vδ1+细胞再次代表主要的γδ T细胞类型。这一发现表明，事实上，Vγ6/Vδ1+亚群的反应并不依赖于细菌或细菌产物的存在。触发Vγ6/Vδ1 γδ T细胞反应的刺激似乎既不是外来的，也不是器官特异性的，而是由炎症过程中诱导产生的自身来源的宿主抗原或信号组成。
