Mukasa A, Lahn M, Pflum E K, Born W, O'Brien R L
National Jewish Medical and Research Center, Denver, CO 80206, USA.
J Immunol. 1997 Dec 15;159(12):5787-94.
Inflammatory responses are induced in both testes of a mouse following injection of Listeria monocytogenes into one testis. Although the uninjected testis contains no detectable bacteria, it undergoes an autoimmune attack. Normally, the testis lacks lymphocytes, but in the infected and autoimmune state, both gamma delta and alpha beta T cells are found as infiltrates. Here, we have examined the repertoire of the infiltrating gamma delta T cells, using two different methods, and found a high frequency of V gamma 6/V delta 1 gamma delta T cells in both infected and autoimmune testes. All of these expressed the invariant V gamma 6/V delta 1 TCR previously reported. However, secondary gamma and delta transcripts present within V gamma 6/V delta 1 hybridomas indicated nonclonality. Interestingly, some of these secondary transcripts were derived from gamma gene rearrangements not previously found in this gamma delta T cell subset, implying a difference in its origin. The increase in V gamma 6/V delta 1 cells observed here in both infected and autoimmune testes, together with our previous finding of a preferential response by the same subset in Listeria-infected liver, indicates that their response is triggered by the inflammation rather than by the infectious agent or because they are already resident in the tissue. We and others have previously reported that the presence of gamma delta T cells during certain inflammatory conditions correlates with less host tissue damage. This result, together with the evidence presented here, further implies that a response by the V gamma 6/V delta 1 subset in some way exerts a controlling influence on the host inflammatory response.
将单核细胞增生李斯特菌注射到小鼠的一侧睾丸后，两侧睾丸均会引发炎症反应。尽管未注射的睾丸中未检测到细菌，但它会遭受自身免疫攻击。正常情况下，睾丸中缺乏淋巴细胞，但在感染和自身免疫状态下，均可发现γδ和αβ T细胞浸润。在此，我们使用两种不同方法检测了浸润的γδ T细胞库，发现在感染和自身免疫的睾丸中，Vγ6/Vδ1 γδ T细胞的频率都很高。所有这些细胞均表达先前报道的恒定Vγ6/Vδ1 TCR。然而，Vγ6/Vδ1杂交瘤中存在的二级γ和δ转录本表明其具有非克隆性。有趣的是，其中一些二级转录本源自此前在该γδ T细胞亚群中未发现的γ基因重排，这意味着其起源存在差异。我们在此观察到，在感染和自身免疫的睾丸中Vγ6/Vδ1细胞均增加，以及我们之前发现同一亚群在李斯特菌感染的肝脏中具有优先反应，这表明它们的反应是由炎症触发的，而非由感染因子触发，也不是因为它们已驻留在组织中。我们和其他人之前曾报道，在某些炎症条件下γδ T细胞的存在与较少的宿主组织损伤相关。这一结果，连同此处提供的证据，进一步表明Vγ6/Vδ1亚群的反应以某种方式对宿主炎症反应施加了控制影响。
