Norwood D E, Gilmour A
Department of Food Science (Food Microbiology), Queen's University of Belfast, UK.
J Appl Microbiol. 1999 Apr;86(4):576-82. doi: 10.1046/j.1365-2672.1999.00694.x.
An assay was developed to measure the number of Listeria monocytogenes cells adhering to stainless steel, and was used to investigate the adherence of 111 strains of the organism, which included representatives with respect to serotype, carriage of plasmids, source and persistence in the food processing environment. Growth and adherence curves of four L. monocytogenes strains over 48 h were obtained. While the growth curves of all four micro-organisms were seen to reach similar levels at stationary phase, there was still substantial variation among the adherence curves. In addition, a scatter-graph of growth vs adherence counts at 24 h showed poor correlation. These factors indicated that interstrain variation in adherence at stationary phase is due to factor(s) intrinsic to each strain of L. monocytogenes. Persistent strains were found to adhere in significantly greater numbers than sporadic strains, and variation was also found among serotypes, with serotype 1/2c showing significantly greater adherence than serotypes 1/2a and 4b; 4b strains were significantly higher than those of 1/2a strains. No significant difference was found between strains according to source or plasmid carriage.
开发了一种测定方法来测量粘附在不锈钢上的单核细胞增生李斯特菌细胞数量，并用于研究该生物体111个菌株的粘附情况，这些菌株包括血清型、质粒携带、来源以及在食品加工环境中的持久性方面的代表菌株。获得了4株单核细胞增生李斯特菌在48小时内的生长和粘附曲线。虽然所有4种微生物的生长曲线在稳定期都达到了相似水平，但粘附曲线之间仍存在很大差异。此外，24小时时生长与粘附计数的散点图显示相关性较差。这些因素表明，稳定期菌株间粘附的差异是由于单核细胞增生李斯特菌各菌株固有的因素所致。发现持续存在的菌株比散发菌株的粘附数量显著更多，并且在血清型之间也发现了差异，血清型1/2c的粘附显著高于血清型1/2a和4b；4b菌株显著高于1/2a菌株。根据来源或质粒携带情况，菌株之间未发现显著差异。
