Norwood D E, Gilmour A
Department of Food Science (Food Microbiology), The Queen's University of Belfast, Newforge Lane, Belfast BT9 5PX, N. Ireland, UK.
Lett Appl Microbiol. 2001 Oct;33(4):320-4. doi: 10.1046/j.1472-765x.2001.01004.x.
To determine the differential adherence capabilities at three different temperatures of Listeria monocytogenes Scott A, a clinical food pathogen, and L. monocytogenes FM876, a persistent strain from a milk-processing environment, to stainless steel.
Differential adherence was investigated by submerging stainless steel coupons in both 48-h Listeria monocultures and mixed cultures additionally containing Staphylococcus xylosus DP5H and Pseudomonas fragi ATCC 4973. Immunofluorescent microscopy and image analysis techniques were utilized to identify and quantify the L. monocytogenes cells adhering to the steel at 4 degrees C, 18 degrees C and 30 degrees C. The monoculture biofilms consistently contained greater L. monocytogenes numbers than the multispecies biofilms, with the persistent strain FM876 showing significantly greater adherence than strain Scott A. Optimum adherence occurred at 18 degrees C in monoculture biofilms.
L. monocytogenes strains exhibit differential, temperature-dependent, adherence to stainless steel.
These results demonstrate temperature dependent biofilm adherence and support previous findings that persistent strains exhibit increased adherence capability.
确定临床食源性病原体单核细胞增生李斯特菌斯科特A株以及来自牛奶加工环境的持久菌株单核细胞增生李斯特菌FM876在三种不同温度下对不锈钢的差异黏附能力。
通过将不锈钢试片浸入48小时的单核细胞增生李斯特菌单培养物以及另外含有木糖葡萄球菌DP5H和脆弱拟杆菌ATCC 4973的混合培养物中来研究差异黏附。利用免疫荧光显微镜和图像分析技术来识别和量化在4℃、18℃和30℃下附着在钢上的单核细胞增生李斯特菌细胞。单培养生物膜中的单核细胞增生李斯特菌数量始终多于多物种生物膜，持久菌株FM876的黏附能力明显高于斯科特A株。在单培养生物膜中，18℃时黏附最佳。
单核细胞增生李斯特菌菌株对不锈钢表现出不同的、温度依赖性的黏附。
这些结果证明了温度依赖性生物膜黏附，并支持了先前关于持久菌株表现出增强黏附能力的发现。
