Rodríguez-Argüelles M C, Sánchez A, Belicchi Ferrari M, Gasparri Fava G, Pelizzi C, Pelosi G, Albertini R, Lunghi P, Pinelli S
Departamento de Química Inorgánica, Universidade de Vigo, Spain.
J Inorg Biochem. 1999 Jan-Feb;73(1-2):7-15. doi: 10.1016/s0162-0134(98)10085-5.
Manganese, iron, cobalt, nickel, copper and zinc complexes of isatin-beta-thiosemicarbazone (H2L) have been synthesized and spectroscopically characterized The X-ray crystal structures of two nickel complexes, namely [Ni(HL)2]. EtOH (1) and [Ni(HL)2]. 2DMF (2), reveal a distorted octahedral coordination with the monodeprotonated ligand that behaves as an O,N,S terdentate. Different packing interactions are determined by the presence of different crystallization solvents, i.e., ethanol in 1 and dimethylformamide (DMF) in 2. 1H and 13C NMR studies of the ligand and zinc complexes in solution were carried out and a complete assignment for the ligand was made by homodecoupling, gradient assisted 2D 1H-13C HMQC and HMBC NMR spectroscopy. Biological studies, carried out in vitro on human leukaemic cell lines U937, have shown that the free ligand and the copper (II) complex are more active in the inhibition of cell proliferation than the nickel complexes. No compound was able to induce apoptosis.
已合成了异吲哚酮-β-硫代半卡巴腙（H2L）的锰、铁、钴、镍、铜和锌配合物，并通过光谱对其进行了表征。两种镍配合物，即[Ni(HL)2].EtOH（1）和[Ni(HL)2].2DMF（2）的X射线晶体结构显示，与单去质子化配体形成扭曲的八面体配位，该配体表现为O、N、S三齿配体。不同的结晶溶剂（即1中的乙醇和2中的二甲基甲酰胺（DMF））决定了不同的堆积相互作用。对溶液中的配体和锌配合物进行了1H和13C NMR研究，并通过同核去耦、梯度辅助二维1H-13C HMQC和HMBC NMR光谱对配体进行了完全归属。对人白血病细胞系U937进行的体外生物学研究表明，游离配体和铜（II）配合物在抑制细胞增殖方面比镍配合物更具活性。没有化合物能够诱导细胞凋亡。
