Rodríuez-Argüelles Maria C, Belicchi errari Marisa, Bisceglie Franco, Pelizzi Corrado, Pelosi Giorgio, Pinelli Silvana, Sassi Monica
Departamento de Quíica Inorgánica, Universidade de Vigo, 36200 Vigo, Spain.
J Inorg Biochem. 2004 Feb;98(2):313-21. doi: 10.1016/j.jinorgbio.2003.10.006.
Nickel, copper, and zinc complexes of isatin (H(2)L(1)) and N-methylisatin 3-picolinoyl hydrazone (HL(2)), were synthesized and characterized by means of spectroscopic techniques. H(2)L(1) and a nickel complex [Ni(L(2))(2)].2C(6)H(14) were also characterized by X-ray diffractometry. Biological studies, carried out in vitro on human leukemic cell lines TOM 1 and NB4, have shown that both ligands and some copper and nickel complexes are active in inhibiting cell proliferation. Compounds H(2)L(1), Cu(HL(1))(2).2H(2)O, Zn(HL(1))(2).2H(2)O inhibit DNA synthesis and act constantly with time between 0 and 72 h. The cell cycle analysis has highlighted a reduction in the number of cells in phase S of about 40%. The same compounds present only a precocious action on cell line NB4 and therefore their activity is cell target specific.
合成了异吲哚酮（H₂L₁）和N - 甲基异吲哚酮3 - 吡啶甲酰腙（HL₂）的镍、铜和锌配合物，并通过光谱技术对其进行了表征。H₂L₁和镍配合物[Ni(L₂)₂]·2C₆H₁₄也通过X射线衍射法进行了表征。在人白血病细胞系TOM 1和NB4上进行的体外生物学研究表明，配体以及一些铜和镍配合物在抑制细胞增殖方面具有活性。化合物H₂L₁、Cu(HL₁)₂·2H₂O、Zn(HL₁)₂·2H₂O抑制DNA合成，并在0至72小时内随时间持续发挥作用。细胞周期分析突出显示，S期细胞数量减少了约40%。相同的化合物对细胞系NB4仅呈现早熟作用，因此它们的活性具有细胞靶点特异性。
