Fukuyama Y, Yoshida S, Yanagisawa S, Shimizu M
Department of Oral and Maxillo-Facial Surgery, Oita Medical University, Japan.
Biospectroscopy. 1999;5(2):117-26. doi: 10.1002/(SICI)1520-6343(1999)5:2<117::AID-BSPY5>3.0.CO;2-K.
We investigated the differences of Fourier transform infrared (FTIR) spectra between oral squamous cell carcinoma (OSCC) and normal gingival epithelium (NGE) or normal subgingival tissue (NST). We used 15 specimens of OSCC which had not been treated before measurement and 10 of NGE or NST. We also used cultured oral squamous cell carcinoma (COSCC) and the tissue (MSCC) which massed for 3 months after the cultured oral squamous cell carcinoma was transplanted into the lower back of a rat. Those tissue spectra were compared with the purified human collagens and human keratin. One half of every tissue specimen was measured with FTIR and the other half was investigated histologically. The differences of FTIR spectra between OSCC and NGE were observed in the bands between 1431 and 1482 cm(-1) and between 1183 and 1274 cm(-1). The shoulder at 1368 cm(-1) tended to disappear in OSCC, and the peaks at 1246 and 1083 cm(-1) found in NGE tended to shift to those at 1242 and 1086 cm(-1) in OSCC, respectively. The infrared spectrum of NST was noticed to be strongly influenced by the presence of collagen. Significant differences were also observed in the second derivative FTIR spectra between OSCC and NGE. Our data suggested that this infrared technique is applicable to clinical diagnostics.
我们研究了口腔鳞状细胞癌（OSCC）与正常牙龈上皮（NGE）或正常龈下组织（NST）之间傅里叶变换红外（FTIR）光谱的差异。我们使用了15个未经治疗的OSCC标本进行测量，以及10个NGE或NST标本。我们还使用了培养的口腔鳞状细胞癌（COSCC）以及将培养的口腔鳞状细胞癌移植到大鼠下背部3个月后聚集形成的组织（MSCC）。将这些组织光谱与纯化的人胶原蛋白和人角蛋白进行比较。每个组织标本的一半用FTIR测量，另一半进行组织学研究。在1431至1482 cm(-1)以及1183至1274 cm(-1)波段观察到OSCC与NGE之间FTIR光谱的差异。OSCC中1368 cm(-1)处的肩峰趋于消失，NGE中1246和1083 cm(-1)处的峰在OSCC中分别倾向于移至1242和1086 cm(-1)处。注意到NST的红外光谱受胶原蛋白存在的强烈影响。在OSCC与NGE之间的二阶导数FTIR光谱中也观察到显著差异。我们的数据表明这种红外技术适用于临床诊断。
