Ladant D, Ullmann A
Dept of Biochemistry and Molecular Genetics, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France.
Trends Microbiol. 1999 Apr;7(4):172-6. doi: 10.1016/s0966-842x(99)01468-7.
Bordetella pertussis secretes a calmodulin-activated adenylate cyclase toxin (CyaA) that is able to deliver its amino-terminal catalytic domain into the cytosol of eukaryotic cells. The novelty of the structural organization and conformational flexibility of the CyaA catalytic domain has opened up the way for exploiting this protein as a tool for several biological applications, including epitope delivery, protein targeting and characterization of protein-protein interactions.
百日咳博德特氏菌分泌一种钙调蛋白激活的腺苷酸环化酶毒素（CyaA），该毒素能够将其氨基末端催化结构域递送至真核细胞的细胞质中。CyaA催化结构域的结构组织和构象灵活性的新颖性为将该蛋白用作多种生物学应用的工具开辟了道路，这些应用包括表位递送、蛋白质靶向以及蛋白质-蛋白质相互作用的表征。
