Nakamura R M, Peebles C L, Tan E M
Am J Clin Pathol. 1978 Nov;70(5):800-7. doi: 10.1093/ajcp/70.5.800.
A reproducible hemagglutination procedure to detect antibodies to Sm and ribonucleoprotein nuclear antigens is described. The application and interpretation is discussed. The hemagglutination test was found to be more sensitive than the immunodiffusion test; however, the hemagglutination test may not detect the presence of low titers of anti-ribonucleoprotein in the presence of a high level of anti-Sm antibody. Anti-ribonucleoprotein antibodies in the presence of high titers of anti-Sm antibodies are best identified by gel precipitation methods where precipitin lines can be characterized by their interaction with precipitin lines produced by known prototype antisera.
本文描述了一种用于检测抗Sm和核糖核蛋白核抗原抗体的可重复血凝程序，并对其应用和解读进行了讨论。发现血凝试验比免疫扩散试验更敏感；然而，在高滴度抗Sm抗体存在的情况下，血凝试验可能无法检测到低滴度抗核糖核蛋白的存在。在高滴度抗Sm抗体存在的情况下，抗核糖核蛋白抗体最好通过凝胶沉淀法来鉴定，其中沉淀线可通过它们与已知原型抗血清产生的沉淀线的相互作用来表征。
