Kozin F, Fowler M
Am J Clin Pathol. 1979 Apr;71(4):437-40. doi: 10.1093/ajcp/71.4.437.
Extractable nuclear antigens have been identified in a number of systemic rheumatic diseases and may be useful in differentiating those with overlapping clinical features. A number of detection systems have been described but technical feasibility and sensitivity have limited widespread application. We describe a simple immunodiffusion method to identify extractable nuclear antigens with high specificity and sensitivity that compares favorably with parallel hemagglutination studies. This method can be applied easily to routine analysis of antinuclear antigens.
在多种系统性风湿性疾病中已鉴定出可提取核抗原，这些抗原可能有助于鉴别具有重叠临床特征的疾病。已经描述了多种检测系统，但技术可行性和敏感性限制了其广泛应用。我们描述了一种简单的免疫扩散方法，该方法可用于鉴定具有高特异性和敏感性的可提取核抗原，与平行血凝试验相比具有优势。该方法可轻松应用于抗核抗原的常规分析。
