Suzuki H, Mori M, Seto K, Kai A, Kawaguchi C, Suzuki M, Suematsu M, Yoneta T, Miura S, Ishii H
Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
Free Radic Biol Med. 1999 Mar;26(5-6):679-84. doi: 10.1016/s0891-5849(98)00248-2.
Helicobacter pylori colonized gastric mucosa is manifest in a significant neutrophil infiltration with an extensive level of oxyradical formation. Mongolian gerbil is one of the excellent models for H. pylori-infection. The present study was designed to investigate pro- and antioxidant formation in the stomach of H. pylori-positive gerbils. Fourteen male Mongolian gerbils (MGS/Sea) were orally inoculated with H. pylori (ATCC43504) (Hp group) and 15 gerbils were inoculated with the culture media (Control). H. pylori infection was confirmed by the serum anti-H. pylori IgG test. Each gerbil was evaluated 6 or 12 weeks after the inoculation. Neutrophil infiltration was assessed by the tissue MPO activity. Mucosal oxidative stress was evaluated by thiobarbituric acid-reactive substances (TBARS), total glutathione contents, glutathione peroxidase (GSHPx) activity and Cu-, Zn-superoxide dismutase (SOD) activity. In Hp group, the H. pylori was persistently infected until 12 weeks. The level of MPO activity was significantly higher in Hp group at 6 and 12 weeks. Although the levels of TBARS and total glutathione were within the same range as controls at 6 weeks, they were significantly increased at 12 weeks. However, GSHPx activity was significantly increased at 6 weeks, but became the same range with the controls at 12 weeks. SOD activity showed no significant increase in Hp group at 6 and 12 weeks. In conclusion, H. pylori inoculation induced gastric mucosal neutrophil activation and pro-oxidant formation and also increased total glutathione contents, one of the mucosal antioxidants in gerbils.
幽门螺杆菌定殖于胃黏膜表现为明显的中性粒细胞浸润以及高水平的氧自由基形成。蒙古沙鼠是幽门螺杆菌感染的优良模型之一。本研究旨在调查幽门螺杆菌阳性沙鼠胃内促氧化剂和抗氧化剂的形成情况。14只雄性蒙古沙鼠（MGS/Sea）经口接种幽门螺杆菌（ATCC43504）（Hp组），15只沙鼠接种培养基（对照组）。通过血清抗幽门螺杆菌IgG检测确认幽门螺杆菌感染情况。接种后6周或12周对每只沙鼠进行评估。通过组织MPO活性评估中性粒细胞浸润情况。通过硫代巴比妥酸反应性物质（TBARS）、总谷胱甘肽含量、谷胱甘肽过氧化物酶（GSHPx）活性和铜锌超氧化物歧化酶（SOD）活性评估黏膜氧化应激。在Hp组，幽门螺杆菌持续感染至12周。Hp组在6周和12周时MPO活性水平显著更高。虽然6周时TBARS和总谷胱甘肽水平与对照组处于同一范围，但在12周时显著升高。然而，GSHPx活性在6周时显著升高，但在12周时与对照组处于同一范围。Hp组在6周和12周时SOD活性无显著增加。总之，接种幽门螺杆菌可诱导胃黏膜中性粒细胞活化和促氧化剂形成，同时也增加了沙鼠黏膜抗氧化剂之一的总谷胱甘肽含量。
