中药雷公藤提取物对角叉菜胶诱导的体内炎症的抑制作用

Suppression of carrageenan-induced inflammation in vivo by an extract of the Chinese herbal remedy Tripterygium wilfordii Hook F.

作者信息

Tao X, Ma L, Mao Y, Lipsky P E

机构信息

Harold C. Simmons Arthritis Research Center, Dallas, TX 75235-8884, USA.

出版信息

Inflamm Res. 1999 Mar;48(3):139-48. doi: 10.1007/s000110050437.

DOI:10.1007/s000110050437

PMID:10219656

Abstract

OBJECTIVE AND DESIGN

The current studies investigated the effect of subacute administration of an ethyl acetate extract of the Chinese herbal remedy, Tripterygium wilfordii Hook F (TWHF) on the inflammatory response induced by carrageenan in the rat.

MATERIALS AND SUBJECTS

F344 rats were randomly divided into 3 groups of seven animals.

TREATMENT

The rats were treated orally for 5 days with either vehicle or an ethyl acetate extract of TWHF at a dose of 270 mg/kg, equivalent to 1/5 of the dose causing death in 50% of the animals.

METHODS

Air pouches were induced subcutaneously on the backs of rats and injected with carrageenan on the last day of treatment. Sixteen hours after carrageenan challenge, the air pouches were removed and analyzed. Student's t-test was employed for statistical analysis.

RESULTS

The number of exudate cells and the concentration of PGE2, nitrite and TNF-alpha in the exudate obtained from TWHF-treated animals were significantly reduced (by 69%, 78%, 57% and 77%, respectively) compared to that from vehicle-treated animals. mRNA for cyclooxygenase-2 was markedly suppressed in the air pouch lining tissue of TWHF treated rats (p < 0.001). In contrast, PGE2 content of the kidney and stomach and the production of PGE2, nitrite and TNF-alpha by spleen cells was not affected by treatment with TWHF.

CONCLUSIONS

These results indicate that TWHF exerts a significant anti-inflammatory activity in vivo.

摘要

目的与设计

当前研究调查了中药雷公藤（Tripterygium wilfordii Hook F，TWHF）乙酸乙酯提取物亚急性给药对角叉菜胶诱导的大鼠炎症反应的影响。

材料与对象

将F344大鼠随机分为3组，每组7只动物。

处理

大鼠口服给予赋形剂或剂量为270 mg/kg的TWHF乙酸乙酯提取物，持续5天，该剂量相当于使50%动物死亡剂量的1/5。

方法

在大鼠背部皮下诱导形成气袋，并在处理的最后一天注射角叉菜胶。角叉菜胶攻击16小时后，取出气袋并进行分析。采用学生t检验进行统计分析。

结果

与赋形剂处理的动物相比，TWHF处理动物的渗出细胞数量以及渗出液中PGE2、亚硝酸盐和TNF-α的浓度显著降低（分别降低69%、78%、57%和77%）。在TWHF处理大鼠的气袋内衬组织中，环氧合酶-2的mRNA明显受到抑制（p < 0.001）。相比之下，肾脏和胃中的PGE2含量以及脾细胞产生的PGE2、亚硝酸盐和TNF-α不受TWHF处理的影响。