Puthavathana P, Horthongkham N, Roongpisuthipong A, Maythangkul P, Kanyok R, Boonsirikhamchai P, Chaiyakul P
Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Asian Pac J Allergy Immunol. 1998 Dec;16(4):177-83.
Papanicolaou (Pap) stain, immunoperoxidase (IP) stain and polymerase chain reaction (PCR) were evaluated against the virus isolation method for their sensitivity and specificity in the diagnosis of herpes simplex virus (HSV) infection in 96 women who were suspected of genital herpes. The result showed that the sensitivity of PCR, IP and Pap stain was 100, 92.0 and 62.7%, respectively, while the specificity was 76.2, 66.7 and 81.0%, respectively. PCR was even more sensitive than the virus isolation technique. As Pap stain is the technique routinely performed for diagnosing genital herpes in most of the hospitals in Thailand, its low sensitivity should be taken into consideration. Based on the investigation by all four techniques together, HSV infection was diagnosed in 91.6% of the cases suspected of genital herpes which reflected higher precision of the clinical diagnosis over Pap stain.
针对96名疑似生殖器疱疹的女性，评估了巴氏（Pap）染色、免疫过氧化物酶（IP）染色和聚合酶链反应（PCR）相对于病毒分离方法在诊断单纯疱疹病毒（HSV）感染方面的敏感性和特异性。结果显示，PCR、IP染色和巴氏染色的敏感性分别为100%、92.0%和62.7%，而特异性分别为76.2%、66.7%和81.0%。PCR甚至比病毒分离技术更敏感。由于巴氏染色是泰国大多数医院常规用于诊断生殖器疱疹的技术，其低敏感性应予以考虑。综合所有四种技术的调查，91.6%疑似生殖器疱疹的病例被诊断为HSV感染，这反映出临床诊断比巴氏染色具有更高的准确性。
