Adams R L, Springall D R, Levene M M, Bushell T E
J Pathol. 1984 Aug;143(4):241-7. doi: 10.1002/path.1711430403.
Herpes simplex virus type 2 is demonstrated in routine cervical smears by immunocytochemistry. Optimum antibody dilution and suitable fixation were determined and immunostaining results were found to compare favourably with those obtained by screening Papanicolaou-stained smears or by virus culture methods (virus isolation). Results obtained for immunostaining of smears from 158 patients agreed with those of virus isolation in 47 of 50 cases where virus isolation was performed. It is suggested that this immunocytochemical technique is valuable in detection of Herpes simplex virus in cervical smears, offering an inexpensive and rapid laboratory diagnosis with advantage to both patient and clinician.
通过免疫细胞化学方法在常规宫颈涂片检查中检测出2型单纯疱疹病毒。确定了最佳抗体稀释度和合适的固定方法，发现免疫染色结果与通过巴氏染色涂片筛查或病毒培养方法（病毒分离）获得的结果相比具有优势。对158例患者涂片进行免疫染色的结果与50例进行病毒分离的病例中的47例病毒分离结果一致。有人认为，这种免疫细胞化学技术在宫颈涂片中检测单纯疱疹病毒方面具有重要价值，它提供了一种廉价且快速的实验室诊断方法，对患者和临床医生都有利。
