van der Maarel M J, Sprenger W, Haanstra R, Forney L J
Laboratory of Microbial Ecology, Center for Ecological and Evolutionary Studies, University of Groningen, Haren, Netherlands.
FEMS Microbiol Lett. 1999 Apr 1;173(1):189-94. doi: 10.1111/j.1574-6968.1999.tb13501.x.
A methanogen-specific nested PCR approach was used to detect methanogenic archaea in seawater particles of the North Sea and the feces and the digestive tract of flounder (Platichthys flesus), a fish found in the North Sea. A number of 16S rDNA sequences with 97.6-99.5% similarity to Methanococcoides methylutens were found in the seawater particles as well as the digestive tract and fecal samples.
采用一种产甲烷菌特异性巢式PCR方法，检测北海海水颗粒以及北海鱼类鲽（欧洲黄盖鲽）粪便和消化道中的产甲烷古菌。在海水颗粒以及消化道和粪便样本中发现了一些与甲基营养型甲烷球菌16S rDNA序列相似度为97.6 - 99.5%的序列。
