Denys A, Kowalska M, Szydłowska T, Białek J
Department of Medical Microbiology, Military Medical Academy, Lódź.
Arch Immunol Ther Exp (Warsz). 1987;35(4):463-71.
Bactericidal activity of mouse peritoneal granulocytes was examined on the 3rd, 6th, 9th and 14th day after infection. 18-hour-old S. aureus 209P culture was used for examination. Human serum of AB group was used for opsonization. The mixture of bacteria, leukocytes and serum was placed at 37 degrees C for 2 h. Every 30 minutes the samples were taken to estimate the total number of living bacteria and the number of live intracellular bacteria. In the group of animals infected with bacteria as well as in the animals infected with non-adapted influenza viruses, granulocytes showed increased intracellular killing of test bacteria. The decrease of bactericidal activity of granulocytes was observed when they were taken from mice infected with adapted influenza viruses. In mixed infections, with the use of non-adapted virus and bacteria, the decrease of bactericidal activity of granulocytes was observed on the 6th and 9th day after infection. In the group of animals infected simultaneously with non-adapted virus and bacteria, increased bactericidal activity of granulocytes was observed in the initial period of infection. At the same time, on the 14th day this ability significantly decreased.
在感染后的第3天、第6天、第9天和第14天检测了小鼠腹腔粒细胞的杀菌活性。使用18小时龄的金黄色葡萄球菌209P培养物进行检测。使用AB型人血清进行调理作用。将细菌、白细胞和血清的混合物置于37℃下2小时。每隔30分钟取样以估计活菌总数和活细胞内细菌数。在感染细菌的动物组以及感染非适应性流感病毒的动物中，粒细胞对测试细菌的细胞内杀伤作用增强。当从感染适应性流感病毒的小鼠中获取粒细胞时，观察到粒细胞杀菌活性降低。在混合感染中，使用非适应性病毒和细菌时，在感染后第6天和第9天观察到粒细胞杀菌活性降低。在同时感染非适应性病毒和细菌的动物组中，在感染初期观察到粒细胞杀菌活性增强。同时，在第14天，这种能力显著下降。
