Siennicka J, Litwińska B, Seferyńska I, Pałynyczko G
Zakład Wirusologii PZH w Warszawie.
Med Dosw Mikrobiol. 1998;50(3-4):285-92.
The purpose of this paper was to estimate the best method of CMV diagnosis in leukemic patients. Materials from 9 patients (serum, heparinized blood and urine) were investigated by serological methods (ELISA and Western blot), for the presence of specific antigens and virus capable of replication, and also by genetic methods (hybridization and PCR). It seems, that the best method for CMV diagnosis in leukemic patients is hybridization performed in quantitation variant, and that DNA CMV level of approximately 20 pg/ml of blood was not linked to symptomatic infection.
本文的目的是评估白血病患者中巨细胞病毒(CMV)诊断的最佳方法。采用血清学方法(酶联免疫吸附测定法和蛋白质印迹法)对9例患者的材料(血清、肝素抗凝血和尿液)进行检测,以确定是否存在特异性抗原和具有复制能力的病毒,同时采用遗传学方法(杂交和聚合酶链反应)进行检测。似乎,白血病患者CMV诊断的最佳方法是定量变体杂交,且血液中CMV DNA水平约为20 pg/ml与症状性感染无关。