Siennicka J, Durlik M, Litwińska B, Chmura A, Lewandowska D, Lao M, Paczek L, Kańtoch M
Department of Virology, National Institute of Hygiene, Warsaw, Poland.
Acta Microbiol Pol. 1999;48(1):61-71.
Early diagnosis of CMV infection is very important mainly in transplant recipients because CMV infection is a frequent complication after transplantation. In this work we compared different laboratory methods: ELISA (IgG, IgM), Western blot,shell vial, antigenemia assay (pp65), the immunofluorescent method with epithelial cells from urine (IF), DNA in leukocytes by PCR and DNA in leukocytes by hybridization (HCS) to estimate the most proper method for diagnosis of CMV in renal transplant recipients. This preliminary study showed that HCS, PCR and Western blot are sensitive methods for detecting CMV infection. Using HCS in quantitative variant we obtained a very good correlation between DNA load and clinical symptoms.
巨细胞病毒（CMV）感染的早期诊断主要对移植受者非常重要，因为CMV感染是移植后常见的并发症。在这项研究中，我们比较了不同的实验室方法：酶联免疫吸附测定（ELISA，检测IgG、IgM）、蛋白质印迹法、空斑试验、抗原血症检测（pp65）、尿液上皮细胞免疫荧光法（IF）、白细胞DNA的聚合酶链反应（PCR）检测以及白细胞DNA杂交检测（HCS），以评估肾移植受者中诊断CMV的最适宜方法。这项初步研究表明，HCS、PCR和蛋白质印迹法是检测CMV感染的敏感方法。使用定量变体的HCS方法，我们发现DNA载量与临床症状之间具有很好的相关性。
