Filice G A, Clabots C R, Riciputi P E, Goñi-Laguardia O, Pomeroy C
Infectious Disease Section, Veterans Affairs Medical Center, Minneapolis, Minnesota, USA.
Infect Immun. 1999 May;67(5):2082-9. doi: 10.1128/IAI.67.5.2082-2089.1999.
We studied cytokine proteins and mRNAs in mice with two forms of Toxoplasma gondii pneumonia resulting from reactivation of infection. In the first form, mice were infected with T. gondii, developed and recovered from systemic disease, and then developed pneumonia 3 weeks later. As pulmonary inflammation developed, levels of cytokine mRNAs for gamma interferon (IFN-gamma), interleukin-2 (IL-2), IL-4, and IL-10 increased in bronchoalveolar lavage (BAL) cells or lung tissue, and the level of IFN-gamma protein increased in BAL fluid. The second form of pneumonia occurred as a complication of primary cytomegalovirus (CMV) disease in mice with dormant T. gondii infection. During CMV disease, IL-2 mRNA levels decreased in lung tissue, IL-10 protein levels increased in lung tissue, and IL-10 protein levels increased in BAL fluid. As the mice recovered from CMV disease, T. gondii infection was reactivated in the lungs and was manifested as T. gondii pneumonia. During CMV-induced T. gondii pneumonia, IFN-gamma, IL-2, IL-4, and IL-10 mRNA levels increased in BAL cells or lung tissue, and both IFN-gamma and IL-2 protein levels increased in BAL fluid. We concluded that both forms of T. gondii pneumonia are accompanied by increases in both type 1 T-helper and type 2 T-helper cytokine levels in lungs. The mechanism of CMV-induced reactivation of T. gondii infection in lungs may involve local decreases in IL-2 levels and/or increases in IL-10 levels.
我们研究了因感染再激活而导致的两种形式的弓形虫肺炎小鼠体内的细胞因子蛋白和信使核糖核酸。在第一种形式中，小鼠感染弓形虫，患全身性疾病并康复，然后在3周后患上肺炎。随着肺部炎症的发展，支气管肺泡灌洗（BAL）细胞或肺组织中γ干扰素（IFN-γ）、白细胞介素-2（IL-2）、IL-4和IL-10的细胞因子信使核糖核酸水平升高，BAL液中IFN-γ蛋白水平升高。第二种形式的肺炎发生在患有潜伏性弓形虫感染的小鼠原发性巨细胞病毒（CMV）疾病的并发症中。在CMV疾病期间，肺组织中IL-2信使核糖核酸水平下降，肺组织和BAL液中IL-10蛋白水平升高。随着小鼠从CMV疾病中康复，肺部的弓形虫感染被重新激活，并表现为弓形虫肺炎。在CMV诱导的弓形虫肺炎期间，BAL细胞或肺组织中IFN-γ、IL-2、IL-4和IL-10信使核糖核酸水平升高，BAL液中IFN-γ和IL-2蛋白水平均升高。我们得出结论，两种形式的弓形虫肺炎都伴随着肺部1型辅助性T细胞和2型辅助性T细胞细胞因子水平的升高。CMV诱导肺部弓形虫感染再激活的机制可能涉及局部IL-2水平降低和/或IL-10水平升高。
