Klemm A, Tschernig T, Krug N, Pabst R
Department of Functional and Applied Anatomy, Medical School of Hannover, Germany.
Clin Exp Immunol. 1998 Aug;113(2):252-7. doi: 10.1046/j.1365-2249.1998.00657.x.
Lymphocytes play an important immunoregulatory role in pulmonary immune responses. By releasing cytokines they can control the cell-cell communication of other participating cells. Although it is well established that the lung lymphocytes, localized in distinct compartments, differ in their subset composition, little is known about cytokine production in these compartments during immune responses. Lewis rats were immunized by intravenous administration of sheep erythrocytes on day 0 and day 7 and challenged intratracheally with sheep erythrocytes on day 10. Four days after intratracheal (i.t.) challenge the composition of lymphocyte subsets (CD2+, CD4+, CD8+, B cells, natural killer (NK) cells) in the spleen, blood, lung perfusate, lung tissue and bronchoalveolar lavage fluid (BALF) was characterized, and intracellular IFN-gamma was detected in these subsets by flow cytometry. Comparing control and immunized animals, no changes were found in lymphocyte numbers, subsets or the percentage of IFN-gamma-producing lymphocytes in the spleen, blood and lung perfusate. In lung tissue and BALF, however, the absolute number of all lymphocyte subsets and the percentage of IFN-gamma-producing lymphocytes were increased. When the lymphocyte subsets were analysed an increased percentage of IFN-gamma-producing T cells was found in lung tissue (4.5 +/- 0.6% versus 12.8 +/- 1.1%) and in BALF (7.8 +/- 1.4% versus 14.8 +/- 1.9%) of immunized animals opposed to controls, this increase being seen in both CD4+ and CD8+ cells. Thus, there is an accumulation of T cells with an increased potential to produce IFN-gamma in the lung interstitium and the bronchoalveolar space during pulmonary immune responses.
淋巴细胞在肺部免疫反应中发挥着重要的免疫调节作用。通过释放细胞因子，它们可以控制其他参与细胞之间的细胞通讯。尽管已经明确，位于不同区域的肺淋巴细胞在亚群组成上存在差异，但对于免疫反应期间这些区域中细胞因子的产生情况却知之甚少。在第0天和第7天通过静脉注射绵羊红细胞对Lewis大鼠进行免疫，并在第10天经气管内用绵羊红细胞进行攻击。气管内攻击4天后，对脾脏、血液、肺灌洗液、肺组织和支气管肺泡灌洗液（BALF）中淋巴细胞亚群（CD2 +、CD4 +、CD8 +、B细胞、自然杀伤（NK）细胞）的组成进行了表征，并通过流式细胞术在这些亚群中检测细胞内干扰素-γ。比较对照动物和免疫动物，在脾脏、血液和肺灌洗液中淋巴细胞数量、亚群或产生干扰素-γ的淋巴细胞百分比均未发现变化。然而，在肺组织和BALF中，所有淋巴细胞亚群的绝对数量以及产生干扰素-γ的淋巴细胞百分比均增加。当对淋巴细胞亚群进行分析时，发现与对照相比，免疫动物的肺组织（4.5±0.6%对12.8±1.1%）和BALF（7.8±1.4%对14.8±1.9%）中产生干扰素-γ的T细胞百分比增加，这种增加在CD4 +和CD8 +细胞中均可见。因此，在肺部免疫反应期间，肺间质和支气管肺泡空间中具有增加的产生干扰素-γ潜力的T细胞会发生积聚。
