Karanikas G, Schmaljohann J, Rodrigues M, Chehne F, Granegger S, Sinzinger H
Department of Nuclear Medicine, University of Vienna, Austria.
Thromb Res. 1999 Apr 15;94(2):111-5. doi: 10.1016/s0049-3848(98)00204-7.
Oxine, tropolonate, and mercaptopyridine-N-oxide (MPO) have been widely used with 111In for platelet labeling. The use of positron emission tomography (PET) radioisotopes for platelet labeling would offer a higher sensitivity and resolution over conventional imaging. The medium half-life PET radioisotope 55Co (t1/2 = 18.2 hours) would enable quantitative uptake and cell kinetic studies with radiolabeled platelets. To identify the optimal complex for radiolabeling of platelets with 55Co, we investigated the platelet uptake of 57Co-oxine, 57Co-tropolonate, and 57Co-MPO in varying different parameters. The platelet uptake of Co-complexes was generally low (5-12%), dependent on time and temperature of incubation and density of platelets but independent of the amount of radioactivity. These findings are not promising for potential PET application.
8-羟基喹啉、托酚酮和巯基吡啶-N-氧化物（MPO）已与铟-111广泛用于血小板标记。使用正电子发射断层扫描（PET）放射性同位素进行血小板标记将比传统成像提供更高的灵敏度和分辨率。中等半衰期的PET放射性同位素钴-55（t1/2 = 18.2小时）将能够对放射性标记的血小板进行定量摄取和细胞动力学研究。为了确定用钴-55对血小板进行放射性标记的最佳配合物，我们在不同参数下研究了钴-57-8-羟基喹啉、钴-57-托酚酮和钴-57-MPO的血小板摄取情况。钴配合物的血小板摄取率通常较低（5%-12%），取决于孵育时间和温度以及血小板密度，但与放射性活度无关。这些发现对于PET的潜在应用而言前景不佳。
