Yano Y, Budinger T F, Ebbe S N, Mathis C A, Singh M, Brennan K M, Moyer B R
J Nucl Med. 1985 Dec;26(12):1429-37.
Generator produced 68Ga-labeled platelets could be useful for positron emission tomographic (PET) studies of thrombosis or atherosclerosis. To label platelets with 68Ga, we have studied the effects of trace metals in elutions of 68Ga from 68Ge. Studies were conducted on the formation of lipophilic 68Ga complexes 8-hydroxyquinoline, tropolone, and mercaptopyridine-N-oxide (MPO). Parameters such as pH, buffers, concentration of ligand, and stability with time were investigated. High performance liquid chromatography and instant thin layer chromatography were used to quantitate formation of the 68Ga complex. Platelets from human, dog, and rabbit plasma were incubated with the 68Ga complexes and the percent labeling determined. Accumulation of platelets in the catheter scraped aorta of the rabbit was determined by PET imaging, tissue counting, and autoradiography. Gallium-68 MPO gave 40-60% labeling of rabbit platelets with higher accumulation in the scraped aorta compared to the normal.
发生器产生的68Ga标记血小板可用于血栓形成或动脉粥样硬化的正电子发射断层扫描（PET）研究。为了用68Ga标记血小板，我们研究了痕量金属对68Ga从68Ge洗脱的影响。研究了亲脂性68Ga配合物8-羟基喹啉、托酚酮和巯基吡啶-N-氧化物（MPO）的形成。研究了pH值、缓冲液、配体浓度和随时间的稳定性等参数。采用高效液相色谱法和即时薄层色谱法对68Ga配合物的形成进行定量。将来自人、狗和兔血浆的血小板与68Ga配合物孵育，并测定标记百分比。通过PET成像、组织计数和放射自显影术测定兔导管刮除主动脉中血小板的积聚情况。与正常情况相比，68Ga MPO对兔血小板的标记率为40%-60%，在刮除的主动脉中的积聚更高。
