Chung J G
Department of Medicine, China Medical College, Taichung, Taiwan, Republic of China.
Drug Chem Toxicol. 1999 May;22(2):343-58. doi: 10.3109/01480549909017839.
Diallyl sulfide (DAS) and diallyl disulfide (DADS) were used to determine viability and inhibition of arylamine N-acetyltransferase (NAT) activity in human bladder tumor cells. The NAT activity was measured by high performance liquid chromatography assaying for the amounts of N-acetyl-2-aminofluorene (2-AAF) and N-acetyl-p-aminobenzoic acid (N-Ac-PABA) and remaining 2-aminofluorene (2-AF) and p-aminobenzoic acid (PABA). The viability, NAT activity and 2-AAF-DNA adduct formation in human bladder tumor cells was inhibited by DAS and DADS in a dose-dependent manner, i.e. the higher the concentration of DAS and DADS, the higher the inhibition of NAT activity and cell death. The data also indicated that DAS and DADS decrease the apparent values of Km and Vmax from human bladder tumor cells in both systems examined. This report is the first demonstration to show garlic components did affect human bladder tumor cell NAT activity.
烯丙基硫醚（DAS）和二烯丙基二硫醚（DADS）用于测定人膀胱肿瘤细胞的活力以及对芳胺N - 乙酰基转移酶（NAT）活性的抑制作用。通过高效液相色谱法测定N - 乙酰基 - 2 - 氨基芴（2 - AAF）和N - 乙酰基 - 对氨基苯甲酸（N - Ac - PABA）的量以及剩余的2 - 氨基芴（2 - AF）和对氨基苯甲酸（PABA）来测量NAT活性。DAS和DADS以剂量依赖性方式抑制人膀胱肿瘤细胞中的活力、NAT活性和2 - AAF - DNA加合物形成，即DAS和DADS的浓度越高，对NAT活性的抑制和细胞死亡程度越高。数据还表明，在两个检测系统中，DAS和DADS均降低了人膀胱肿瘤细胞中Km和Vmax的表观值。本报告首次证明大蒜成分确实会影响人膀胱肿瘤细胞的NAT活性。
