大蒜成分二烯丙基硫醚和二烯丙基二硫醚对人早幼粒细胞白血病细胞中芳胺N-乙酰基转移酶活性及2-氨基芴-DNA加合物的影响。

Effects of garlic components diallyl sulfide and diallyl disulfide on arylamine N-acetyltransferase activity and 2-aminofluorene-DNA adducts in human promyelocytic leukemia cells.

作者信息

Lin J G, Chen G W, Su C C, Hung C F, Yang C C, Lee J H, Chung J G

机构信息

Institute of Chinese Medical Science, China Medical College, Taichung, Taiwan, ROC.

出版信息

Am J Chin Med. 2002;30(2-3):315-25. doi: 10.1142/S0192415X02000338.

DOI:10.1142/S0192415X02000338

PMID:12230020

Abstract

Two components of garlic, diallyl sulfide (DAS) and diallyl disulfide (DADS), inhibited arylamine N-acetyltransferase (NAT) activity and 2-aminofluorene-DNA adduct in human promyelocytic leukemia cells (HL-60). The NAT activity was measured by high performance liquid chromatography assaying for amounts of N-acetyl-2-aminofluorene (2-AAF) and remaining 2-aminofluorene (2-AF). Cellular cytosols and intact cell suspensions were assayed. The inhibition of NAT activity and 2-AF-DNA adduct formation in human leukemia cells by DAS and DADS were dose-dependent and were directly proportional. The data also indicated that DAS and DADS decrease the apparent values of Km and Vmax from human leukemia cells in both assays. This is the first report of garlic components affecting human leukemia cell NAT activity and 2-AF-DNA adduct formation.

摘要

大蒜的两种成分，二烯丙基硫醚（DAS）和二烯丙基二硫醚（DADS），抑制了人早幼粒细胞白血病细胞（HL - 60）中的芳胺N - 乙酰转移酶（NAT）活性以及2 - 氨基芴 - DNA加合物的形成。通过高效液相色谱法测定N - 乙酰 - 2 - 氨基芴（2 - AAF）和剩余的2 - 氨基芴（2 - AF）的量来测量NAT活性。对细胞溶质和完整细胞悬液进行了测定。DAS和DADS对人白血病细胞中NAT活性和2 - AF - DNA加合物形成的抑制作用呈剂量依赖性且成正比。数据还表明，在两种测定中，DAS和DADS均降低了人白血病细胞中Km和Vmax的表观值。这是关于大蒜成分影响人白血病细胞NAT活性和2 - AF - DNA加合物形成的首次报道。