Hong Y S, Ham Y A, Choi J H, Kim J
Department of Biochemistry, Medical Research Center, College of Medicine, Ewha Womans University, Seoul, Korea.
Exp Mol Med. 2000 Sep 30;32(3):127-34. doi: 10.1038/emm.2000.22.
Allyl sulfur compounds play a major role in the chemoprevention against carcinogenesis. The present study compared the antiproliferative effects of diallyl sulfide (DAS), diallyl disulfide (DADS) and garlic extract on p53-wild type H460 and p53-null type H1299 non small cell lung cancer cells (NSCLC). The DAS and DADS treatment of both H460 and H1299 cells resulted in the highest numbers of cells in apoptotic state as measured by acridine orange staining, however, garlic extract treatment did not induce any significant apoptotic cells by MTT assay. DADS was found to be more effective in inducing apoptosis on NSCLC. The level of p53 protein in H460 cell was increased following DADS treatment. DAS and garlic extract treatment of H460 cells induced a rise in the level of Bax and a fall of Bcl-2 level. These results demonstrate that DAS, DADS and garlic extract are effective in reduction of anti-proliferative gene in NSCLC and suggest that modulation of apoptosis-associated cellular proteins by DAS, DADS and garlic extract may be the mechanism for apoptosis which merit further investigation as potential chemoprevention agents.
烯丙基硫化合物在癌症化学预防中发挥着重要作用。本研究比较了二烯丙基硫醚（DAS）、二烯丙基二硫醚（DADS）和大蒜提取物对p53野生型H460和p53缺失型H1299非小细胞肺癌细胞（NSCLC）的抗增殖作用。通过吖啶橙染色测定，DAS和DADS对H460和H1299细胞的处理导致处于凋亡状态的细胞数量最多，然而，通过MTT法检测，大蒜提取物处理未诱导出任何显著的凋亡细胞。发现DADS在诱导NSCLC细胞凋亡方面更有效。DADS处理后，H460细胞中p53蛋白水平升高。DAS和大蒜提取物处理H460细胞导致Bax水平升高和Bcl-2水平下降。这些结果表明，DAS、DADS和大蒜提取物在降低NSCLC中的抗增殖基因方面是有效的，并且表明DAS、DADS和大蒜提取物对凋亡相关细胞蛋白的调节可能是凋亡的机制，作为潜在的化学预防剂值得进一步研究。
