Chen G W, Chung J G, Hsieh C L, Lin J G
Department of Surgery, Institute of Chinese Medical Science, China Medical College, Taichung, Taiwan, ROC.
Food Chem Toxicol. 1998 Sep-Oct;36(9-10):761-70. doi: 10.1016/s0278-6915(98)00046-5.
Diallyl sulfide (DAS) and diallyl disulfide (DADS), major components of garlic, were used to determine inhibition of arylamine N-acetyltransferase (NAT) activity in a human colon tumour (adenocarcinoma) cell line. Two assay systems were performed, one with cellular cytosols (9000g supernatant), the other with intact bacterial cell suspensions. The NAT activity in a human colon tumour cell line was inhibited by DAS and DADS in a dose-dependent manner in both system: that is, the greater the concentration of DAS and DADS in the reaction, the greater the inhibition of NAT activities in both systems. The data also indicated that DAS and DADS decrease the apparent values of Km and Vmax of NAT enzymes from human colon tumour cells in both systems examined. This is the first report to demonstrate that garlic components do affect human colon tumour cell NAT activity.
大蒜的主要成分二烯丙基硫醚（DAS）和二烯丙基二硫醚（DADS）被用于测定对人结肠肿瘤（腺癌）细胞系中芳胺N - 乙酰转移酶（NAT）活性的抑制作用。进行了两种检测系统，一种使用细胞胞质溶胶（9000g上清液），另一种使用完整的细菌细胞悬液。在这两种系统中，人结肠肿瘤细胞系中的NAT活性均被DAS和DADS以剂量依赖性方式抑制：也就是说，反应中DAS和DADS的浓度越高，两种系统中NAT活性的抑制作用就越强。数据还表明，在两种检测系统中，DAS和DADS均降低了人结肠肿瘤细胞NAT酶的Km和Vmax的表观值。这是第一份证明大蒜成分确实会影响人结肠肿瘤细胞NAT活性的报告。
