Chung J G, Chen G W, Wu L T, Chang H L, Lin J G, Yeh C C, Wang T F
Department of Medicine, China Medical College, Taichung, Taiwan.
Am J Chin Med. 1998;26(3-4):353-64. doi: 10.1142/S0192415X98000397.
Arylamine N-acctyltransferase (NAT) activities with p-aminobenzoic acid (PABA) and 2-aminofluorene (2-AF) were determined in the bacterium Helicobacter pylori collected from peptic ulcer patients. Two assay systems were performed, one with cellular cytosols, the other with intact cell suspensions. Cytosols or suspensions of H. pylori with or without specific concentrations of diallyl sulfide (DAS) or diallyl disulfide (DADS) co-treatment showed different percentages of 2-AF and PABA acetylation. The data indicated that there was decreased NAT activity associated with increased levels of DAS or DADS in H. pylori cytosols and suspensions. Viability studies on H. pylori demonstrated that DAS or DADS elicited dose-dependent bactericide affects on H. pylori cultures. The data also indicated that DAS and DADS decreased the apparent values of K(m) and Vmax of NAT enzyme from H. pylori in both systems examined. This report is the first demonstration that garlic components can affect H. pylori growth and NAT activity.
对从消化性溃疡患者体内分离出的幽门螺杆菌进行了测定，检测其对对氨基苯甲酸（PABA）和2-氨基芴（2-AF）的芳胺N-乙酰基转移酶（NAT）活性。采用了两种检测系统，一种使用细胞胞质溶胶，另一种使用完整细胞悬液。用或不用特定浓度的二烯丙基硫醚（DAS）或二烯丙基二硫醚（DADS）共同处理的幽门螺杆菌胞质溶胶或悬液，显示出不同百分比的2-AF和PABA乙酰化。数据表明，幽门螺杆菌胞质溶胶和悬液中，随着DAS或DADS水平的升高，NAT活性降低。幽门螺杆菌的活力研究表明，DAS或DADS对幽门螺杆菌培养物产生剂量依赖性杀菌作用。数据还表明，在两种检测系统中，DAS和DADS均降低了幽门螺杆菌NAT酶的表观Km值和Vmax值。本报告首次证明大蒜成分可影响幽门螺杆菌的生长和NAT活性。
