Mentzer R K, Smith N D, Pöch G, Dawson D A
Department of Biology/Toxicology, Ashland University, OH 44805, USA.
Drug Chem Toxicol. 1999 May;22(2):359-74. doi: 10.3109/01480549909017840.
The combined osteolathyric effects of beta-aminopropionitrile (beta APN) and penicillamine (PNC) on developing Xenopus embryos were determined and compared with theoretical effects for the dose-addition and independence models. The testing protocol utilized a 1.2-factor matrix design that generated two single chemical and seven mixture concentration-response curves within 36 treatments. Testing was for 96-h with daily solution removal and replacement. The chi 2 goodness-of-fit test was used to compare experimental responses with theoretical responses. Response curve analyses indicated, in general, a combined effect between that expected for dose-addition and that for independence, which is a combined effect typical of chemicals acting dissimilarly.
测定了β-氨基丙腈（βAPN）和青霉胺（PNC）对发育中的非洲爪蟾胚胎的联合致骨畸形作用，并与剂量相加模型和独立作用模型的理论效应进行了比较。测试方案采用1.2因素矩阵设计，在36种处理中生成了两条单一化学物质和七条混合物浓度-反应曲线。测试持续96小时，每天更换溶液。采用卡方拟合优度检验将实验反应与理论反应进行比较。反应曲线分析表明，总体而言，联合效应介于剂量相加模型和独立作用模型预期的效应之间，这是作用方式不同的化学物质典型的联合效应。
