Schedl T B, Donelson J E
Biochim Biophys Acta. 1978 Oct 24;520(3):539-54. doi: 10.1016/0005-2787(78)90140-5.
A collection of random Drosophila melanogaster DNA fragments cloned individually in Escherichia coli was screened for the presence of sequences complementary to the 4 S, 5 S and 5.8 S RNA species produced in the D. melanogaster Kc tissue culture line. Four D. melanogaster DNA fragments were found which possessed sequences complementary to the 4 S RNA species but not complementary to the 5 S or 5.8 S RNA. One such cloned fragment (6.81 kilobase in length) was characterized further. It hybridizes in situ to region 22A-C of the left arm of chromosome 2 and does not contain repetitive sequences detectable by renaturation (cot) analysis. This same region was reported earlier by Steffensen and Wimber (Genetics (1971) 69, 163--178) to hybridize in situ to bulk tRNA extracted from D. melanogaster.
对单独克隆于大肠杆菌中的一系列随机黑腹果蝇DNA片段进行筛选，以寻找与黑腹果蝇Kc组织培养系中产生的4S、5S和5.8S RNA种类互补的序列。发现了四个黑腹果蝇DNA片段，它们具有与4S RNA种类互补的序列，但与5S或5.8S RNA不互补。对其中一个这样的克隆片段（长度为6.81千碱基）进行了进一步表征。它原位杂交到2号染色体左臂的22A-C区域，并且不包含通过复性（cot）分析可检测到的重复序列。Steffensen和Wimber（《遗传学》（1971年）69卷，163 - 178页） earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier earlier 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