果蝇黑腹果蝇编码tRNA Tyr的基因：基因数量测定的定位
The genes coding for tRNA Tyr of Drosophila melanogaster: localization of determination of the gene numbers.
作者信息
Dudler R, Schmidt T, Bienz M, Kubli E
出版信息
Chromosoma. 1981;84(1):49-60. doi: 10.1007/BF00293362.
Transfer RNA(Tyr) (anticodon G psi A) was isolated from Drosophila melanogaster by means of Sepharose 4B, RPC-5, and polyacrylamide gel electrophoresis. The rRNA was iodinated in vitro with Na125 I and hybridized in situ to salivary gland chromosomes from Drosophila. The genes of rRNA(Tyr) were localized in eight regions of the genome by autoradiography. Restriction enzyme analysis of genomic DNA indicated that the haploid Drosophila genome codes for about 23 tRNA(Tyr) genes. The regions 22F and 85A each contain four to five tRNA(Tyr) genes, whereas the regions 28C, 41AB, 42A, 42E, and 56D each contain two to three tRNA(Tyr) genes.
通过琼脂糖4B、RPC-5和聚丙烯酰胺凝胶电泳从黑腹果蝇中分离出了转运RNA（Tyr）（反密码子GψA）。核糖体RNA在体外用Na125I进行碘化，并原位杂交到果蝇唾液腺染色体上。通过放射自显影将核糖体RNA（Tyr）的基因定位在基因组的八个区域。基因组DNA的限制性酶切分析表明，单倍体果蝇基因组编码约23个tRNA（Tyr）基因。22F和85A区域各包含4至5个tRNA（Tyr）基因，而28C、41AB、42A、42E和56D区域各包含2至3个tRNA（Tyr）基因。
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