通过“原位”杂交对黑腹果蝇中天冬氨酸tRNA2基因进行定位。
The localization of tRNAAsp2 genes from Drosophila melanogaster by "in situ" hybridization.
作者信息
Schmidt T, Egg A H, Kubli E
出版信息
Mol Gen Genet. 1978 Sep 8;164(3):249-54. doi: 10.1007/BF00333153.
PMID:101767
Abstract
Transfer RNAAsp2delta was isolated from Drosophila melanogaster by affinity chromatography on concanavalin A-Sepharose. The tRNA was iodinated "in vitro" with Na [125I] and hybridized "in situ" to salivary gland chromosomes from Drosophila. Subsequent autoradiography allowed the localization of the genes for tRNAAsp2 to the left arm of the second chromosome in the regions 29 D and E.
摘要
通过伴刀豆球蛋白A - 琼脂糖亲和层析从黑腹果蝇中分离出天冬氨酸转运核糖核酸2（Transfer RNAAsp2delta）。该转运核糖核酸在体外用碘化钠[125I]进行碘化，并原位杂交到果蝇的唾液腺染色体上。随后的放射自显影使得天冬氨酸转运核糖核酸2（tRNAAsp2）基因定位到第二条染色体左臂的29 D和E区域。
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