位于细胞遗传学位点90BC的黑腹果蝇转移RNA基因簇。

A Drosophila melanogaster transfer RNA gene cluster at the cytogenetic locus 90BC.

作者信息

DeLotto R, Schedl P

出版信息

J Mol Biol. 1984 Nov 15;179(4):587-605. doi: 10.1016/0022-2836(84)90157-8.

Abstract

We report the isolation and characterization of a 31 X 10(3) base-pair DNA segment containing a cluster of Drosophila melanogaster transfer RNA genes from the cytogenic locus 90BC. Seven distinct coding regions have been identified in a 15 X 10(3) base-pair DNA segment. These coding regions contain at least ten tRNA structural genes and include sequences encoding the following tRNAs: tRNAval, tRNAPro, tRNAAla and tRNAThr. We have determined the nucleotide sequence of six of these structural genes and their flanking regions. These genes do not contain intervening sequences nor do they encode the terminal CCA. The tRNA genes from the locus also appear to be functional when assayed in a Xenopus germinal vesicle in vitro transcriptional system.

摘要

我们报道了从细胞遗传学位点90BC分离并鉴定出一段31×10³碱基对的DNA片段，该片段包含一簇黑腹果蝇转移RNA基因。在一个15×10³碱基对的DNA片段中已鉴定出七个不同的编码区。这些编码区包含至少十个tRNA结构基因，包括编码以下tRNA的序列：tRNAval、tRNAPro、tRNAAla和tRNAThr。我们已经确定了其中六个结构基因及其侧翼区域的核苷酸序列。这些基因不包含间隔序列也不编码末端CCA。当在非洲爪蟾生发泡体外转录系统中进行检测时，该位点的tRNA基因似乎也具有功能。