牛肝色氨酸转运核糖核酸的一级结构。
Primary structure of bovine liver tRNATrp.
作者信息
Fournier M, Labouesse J, Dirheimer G, Fix C, Keith G
出版信息
Biochim Biophys Acta. 1978 Nov 21;521(1):198-208. doi: 10.1016/0005-2787(78)90262-9.
Purified tRNATrp from bovine liver, accepting 1700 pmol tryptophan per A260nm unit, was completely digested with pancreatic ribonuclease and T1 ribonuclease. The sequences of the resulting oligonucleotides were determined and the primary structure of the tRNA was deduced. These analyses showed numerous incomplete post-transcriptional modifications, and several positions heterogenously occupied by two different nucleotides, which lead us to think that in bovine liver there exist a mixture of several tRNATrp.
从牛肝脏中纯化得到的tRNATrp，每A260nm单位可接受1700皮摩尔色氨酸，用胰核糖核酸酶和T1核糖核酸酶将其完全消化。测定所得寡核苷酸的序列，并推导tRNA的一级结构。这些分析显示出许多不完全的转录后修饰，以及几个位置被两种不同核苷酸异质占据，这使我们认为在牛肝脏中存在几种tRNATrp的混合物。
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