Keith G, Dirheimer G
Biochim Biophys Acta. 1978 Jan 26;517(1):133-49. doi: 10.1016/0005-2787(78)90041-2.
Highly purified tRNAPhe from rabbit liver, calf liver and bovine liver were completely digested with pancreatic ribonuclease and ribonuclease T1. The oligonucleotides were separated and identified. The tRNAPhe from rabbit liver and calf liver were partially cleaved with ribonuclease T1 or by action of lead acetate. We describe the analyses of the large fragments and the derivation of the primary structure of these mammalian tRNAsPhe.
用胰核糖核酸酶和核糖核酸酶T1对从兔肝、小牛肝和牛肝中高度纯化的苯丙氨酸转运核糖核酸（tRNAPhe）进行完全消化。分离并鉴定了寡核苷酸。用核糖核酸酶T1或通过醋酸铅作用对兔肝和小牛肝中的tRNAPhe进行部分切割。我们描述了对这些哺乳动物苯丙氨酸转运核糖核酸（tRNAsPhe）的大片段的分析及其一级结构的推导。
