Cacan M, Moreau S, Tailliez R
Biochimie. 1978 Sep 29;60(6-7):685-9. doi: 10.1016/s0300-9084(78)80789-5.
Rat liver microsome UDP-glucuronyltransferase and labelled UDP-glucuronic acid were incubated either with P.R.T. or the compounds obtained by the in vitro metabolism of the toxin. Under the same conditions, labelled P.R.T. or its labelled metabolites were incubated with UDP glucuronyltransferase. Radioactive metabolites were produced with Eremofortin C and Eremofortin C alcohol and in each case, were identified as the corresponding beta-glucuronide conjugate. No measurable glucuronidation of P.R.T. or P.R.T. alcohol was observed. The results outlined in this paper show a good correlation between the biological effects and the ability of forming a glucuronide conjugate.
将大鼠肝脏微粒体UDP - 葡萄糖醛酸基转移酶和标记的UDP - 葡萄糖醛酸与P.R.T. 或该毒素体外代谢获得的化合物一起孵育。在相同条件下，将标记的P.R.T. 或其标记的代谢物与UDP葡萄糖醛酸基转移酶一起孵育。Eremofortin C和Eremofortin C醇产生了放射性代谢物，并且在每种情况下，都被鉴定为相应的β - 葡萄糖醛酸共轭物。未观察到P.R.T. 或P.R.T. 醇的可测量的葡萄糖醛酸化。本文概述的结果表明生物效应与形成葡萄糖醛酸共轭物的能力之间具有良好的相关性。
