Mateva V
Vet Med Nauki. 1976;13(8):11-7.
Strain Perego of the sheep pox virus, after 11 passages in lamb testis cultures, was successfully adapted and cultivated in 10 successive passages in a heterologous tissue--secondary cultures of newborn calf kidney. The infected cultures produced a cytopathic effect similar to that observed in a homologous culture, the difference being that part of the degenerated cells came off from the wall of the flask. At the level of its 10th passage the virus exhibited a titer of 10(3)CPE50. The virus was tested for harmlessness on sheep through a subcutaneous inoculation at a dose of 1 cm3 of a 1:10 dilution, and its testing for activity (1:100 dilution) caused an easily tolerated local reaction with a slight rise in the body temperature, the general status of the test animals remaining unaffected.
绵羊痘病毒佩雷戈毒株在羔羊睾丸培养物中传代11次后，成功适应并在新生小牛肾的异源组织——二次培养物中连续传代10次。受感染的培养物产生了与同源培养物中观察到的类似的细胞病变效应，不同之处在于部分退化细胞从培养瓶壁上脱落。在第10代时，该病毒的滴度为10(3)CPE50。通过皮下接种1 cm3的1:10稀释液对该病毒进行绵羊无害性测试，其活性测试（1:100稀释）引起了易于耐受的局部反应，体温略有升高，试验动物的总体状况未受影响。
