Mateva V, Stoĭchev S
Vet Med Nauki. 1975;12(10):18-23.
The strain Perego of the sheep pox virus, used for the production of the ovinized vaccine in this country, was adapted and cultured in tissue cultures of lamb testes. A total of ten passages in succession of the virus were performed with the manifestation of a characteristic cytopathic effect in the cultures. The titer of the virus was found to be within the range of 10(3.5)CPE50, for the initial passages, to 10(4.5)CPE50, for the final passages. The activity and harmlessness of the virus was tested on sheep. The inoculated 14 sheep with one vaccinal dose each, containing 100 CPE50, and two sheep treated with tenfold higher dose of the virus showed that sheep in general tolerate well the rates at which the virus was applied within the range cited. The general state of the sheep did not exhibit deviations, however, the animals' body temperature rose, and there were reactions at the site of the inoculation. Serum-neutralizing antibodies were established in the blood of sheep one month following vaccination.
该国用于生产羊痘疫苗的羊痘病毒佩雷戈毒株，在羔羊睾丸组织培养物中进行了适应和培养。病毒连续传代了十次，培养物中出现了特征性的细胞病变效应。发现病毒滴度在最初传代时为10(3.5)CPE50，到最后传代时为10(4.5)CPE50。在绵羊身上测试了病毒的活性和无害性。给14只绵羊各接种一剂含100 CPE50的疫苗，给另外两只绵羊接种十倍高剂量的病毒，结果表明，一般来说，绵羊对所述范围内的病毒接种率耐受性良好。绵羊的总体状况未出现偏差，然而，动物体温升高，接种部位出现反应。接种疫苗一个月后，绵羊血液中产生了血清中和抗体。
