Lorén I, Björklund A, Lindvall O
Histochemistry. 1977 Jun 8;52(3):223-39. doi: 10.1007/BF00495859.
The effects of high concentrations of magnesium ions in the cryostat and Vibratome procedures for visualization of catecholamine fluorescence in the central nervous system have been investigated. In cryostat sections, obtained from specimens perfused with a formaldehyde and glyoxylic acid containing buffer, the addition of high concentrations of MgSO4 to the perfusion solution enhances the fluorescence intensity and reduces the unspecific background fluorescence and the diffusion of the catecholamine fluorophore. This improves the visualization of all portions of the central catecholamine-containing neurons. Similar effects are obtained in the formaldehyde-Vibratome technique by the introduction of an immersion bath containing MgSO4 after the sectioning procedure. The use of the magnesium perfusion or immersion steps furthermore increases the reproducibility of the Vibratome and cryostat techniques. The paper describes the improved Vibratome and cryostat techniques used in our laboratory.
研究了在低温恒温器和振动切片机程序中高浓度镁离子对中枢神经系统中儿茶酚胺荧光可视化的影响。在从用含甲醛和乙醛酸的缓冲液灌注的标本获得的低温恒温器切片中，向灌注溶液中添加高浓度的MgSO4可增强荧光强度，降低非特异性背景荧光以及儿茶酚胺荧光团的扩散。这改善了中枢含儿茶酚胺神经元所有部分的可视化。在甲醛 - 振动切片机技术中，在切片程序后引入含有MgSO4的浸泡浴也可获得类似效果。此外，使用镁灌注或浸泡步骤可提高振动切片机和低温恒温器技术的可重复性。本文描述了我们实验室使用的改进的振动切片机和低温恒温器技术。
