纯化淋巴细胞和全血体外淋巴细胞刺激试验检测牛布鲁氏菌流产亚种感染的敏感性和特异性比较。
Comparison of sensitivity and specificity of purified lymphocyte and whole-blood in vitro lymphocyte stimulation assays in detection of Brucella abortus infection in cattle.
作者信息
Kaneene J M, Johnson D W, Anderson R K, Muscoplat C C
出版信息
J Clin Microbiol. 1978 Oct;8(4):396-401. doi: 10.1128/jcm.8.4.396-401.1978.
A study was conducted to compare the sensitivity and specificity of purified lymphocyte and whole-blood in vitro lymphocyte stimulation assays in detection of Brucella abortus infection in cattle. Cattle used were infected with B. abortus field strains or strain 19. Peripheral blood was collected, and lymphocytes for the technique. The blood for the whole-blood lymphocyte stimulation assay was diluted 10-fold with RPMI 1640 medium (without additional serum supplement) and cultured. The two tests were run simultaneously, and B. abortus soluble antigen or concanavalin A was added to the cultures. The cultures were incubated for 6 days and assayed for [3H] thymidine incorporation into their DNA. Generally, cultures of the purified lymphocyte stimulation assay had higher counts per minute than those of the whole-blood lymphocyte stimulation assay, but the stimulation ratios for the two tests were comparable. The two assays were comparable in terms of their sensitivity and specificity as applied to detection of brucella infection in cattle.
开展了一项研究，以比较纯化淋巴细胞和全血体外淋巴细胞刺激试验在检测牛布鲁氏菌感染方面的敏感性和特异性。所用牛感染了布鲁氏菌野毒株或19号菌株。采集外周血，并获取用于该技术的淋巴细胞。用于全血淋巴细胞刺激试验的血液用RPMI 1640培养基（无额外血清补充）稀释10倍后进行培养。两项试验同时进行，并向培养物中加入布鲁氏菌可溶性抗原或刀豆球蛋白A。培养物孵育6天，然后检测[3H]胸腺嘧啶核苷掺入其DNA的情况。一般来说，纯化淋巴细胞刺激试验的培养物每分钟计数高于全血淋巴细胞刺激试验的培养物，但两项试验的刺激率相当。就用于检测牛布鲁氏菌感染而言，这两种试验在敏感性和特异性方面相当。
Zotero 插件