Nikitina Z K, Shishkin S S, Debov S S
Vopr Med Khim. 1976 Nov-Dec;22(6):819-25.
sRNA were extracted from postmitochondrial fraction of rat sceletal muscles by the phenol method. Their yield, physico-chemical properties and acceptor activity during postmortal destruction were studied. Within 2 days after the death yield of sRNA was decreased by 18-20% with maintenance of the physico-chemical properties studied. At the same time the acceptor activity (towards 4 amino acids) was distinctly decreased. For various amino acids correlation between a decrease in the acceptor activity and the time of autolysis was different. Three peaks of radioactivity were observed when 14C-phenyl-alanine-sRNA was fractionated on DEAE-Sephadex A-25; two of them were distinctly decreased after 24 hrs autolysis but the third one was maintained almost without alterations. The data obtained suggest that separate types of sRNA are degraded selectively at early steps of postmortal autolysis.
通过苯酚法从大鼠骨骼肌的线粒体后组分中提取小分子核糖核酸（sRNA）。研究了它们在死后分解过程中的产量、理化性质和受体活性。死亡后2天内，sRNA产量下降了18 - 20%，所研究的理化性质保持不变。与此同时，（对4种氨基酸的）受体活性明显降低。对于不同氨基酸，受体活性降低与自溶时间之间的相关性有所不同。当在二乙氨基乙基葡聚糖A - 25（DEAE - Sephadex A - 25）上对14C - 苯丙氨酸 - sRNA进行分级分离时，观察到三个放射性峰；自溶24小时后，其中两个明显降低，但第三个几乎没有变化。所获得的数据表明，在死后自溶的早期阶段，不同类型的sRNA被选择性降解。
