Fomenko P I, Rebrov L B
Vopr Med Khim. 1979 Jul-Aug;25(4):408-14.
RNA synthesis was studied at various postmortal periods in liver and sceletal muscle slices, incubated in Krebs-Ringer solution (pH 7.4) containing NaCl, KCl, CaCl2, KH2PO4, MgSO4, NaHCO3, glucose, normal horse blood serum, penicillin, heparin, mixture of 20 amino acids and 14C-uridine. The higher rate of postmortal inactivation of RNA synthesis was found in liver tissue as compared to muscles; decreased stability of total endogenous RNA from liver tissue was observed under conditions of autolysis. Various dynamics occurred in dissociation of endogenous RNA from sceletal muscles studied at early (6 hrs) and late (24 hrs) postmortal periods and maintained in Krebs-Ringer solution. As shown by analysis of newly formed RNA from liver tissue using disc electrophoresis, synthesis of high molecular RNA (28 S, 18 S) was primarily impaired in cases of postmortal inhibition of the synthesis; liver cells maintained their capacity for the synthesis of low molecular RNA within 3 hrs after death of the animals.
在含有氯化钠、氯化钾、氯化钙、磷酸二氢钾、硫酸镁、碳酸氢钠、葡萄糖、正常马血清、青霉素、肝素、20种氨基酸混合物和14C-尿苷的 Krebs-Ringer 溶液（pH 7.4）中孵育的肝脏和骨骼肌切片，研究了不同死后时期的RNA合成。与肌肉相比，肝脏组织中RNA合成的死后失活率更高；在自溶条件下，观察到肝脏组织中总内源性RNA的稳定性降低。在死后早期（6小时）和晚期（24小时）研究并保存在Krebs-Ringer溶液中的骨骼肌中，内源性RNA的解离呈现出不同的动态变化。通过圆盘电泳分析肝脏组织新形成的RNA表明，在死后合成受抑制的情况下，高分子量RNA（28S、18S）的合成主要受到损害；在动物死亡后3小时内，肝细胞仍保持合成低分子量RNA的能力。
