Naylor S L, Busby L L, Klebe R J
Somatic Cell Genet. 1976 Mar;2(2):93-111. doi: 10.1007/BF01542624.
The essential amino acid requirement of cultured mammalian cells can be satisfied by 19 amino acid derivatives. This finding (a) confirms the results of animal nutritional studies and (b) identifies 19 essential amino acid derivatives and should permit the isolation of a new class of auxotrophic mutants. Five naturally occurring auxotrophic markers have been detected in this survey; namely, inability to utilize cystathionine and citrulline in Chinese hamster ovary (CHO) cells, inability to metabolize citrulline by HTC+hepatoma cells, and confirmation of Eagle's observation that KB cells can utilize homocystine in place of methionine or cystine and D-cystine in place of L-cystine.
培养的哺乳动物细胞对必需氨基酸的需求可由19种氨基酸衍生物满足。这一发现（a）证实了动物营养研究的结果，（b）鉴定出19种必需氨基酸衍生物，并且应该能够分离出一类新的营养缺陷型突变体。在这项研究中检测到了5种天然存在的营养缺陷型标记；即中国仓鼠卵巢（CHO）细胞无法利用胱硫醚和瓜氨酸，HTC +肝癌细胞无法代谢瓜氨酸，以及证实了伊格尔的观察结果，即KB细胞可以利用同型胱氨酸代替甲硫氨酸或胱氨酸，利用D-胱氨酸代替L-胱氨酸。
