Mackall J C, Student A K, Polakis S E, Lane M D
J Biol Chem. 1976 Oct 25;251(20):6462-4.
3T3-L1 fibroblasts differentiate in culture into cells having adipocyte character. This transition is accompanied by a 40- to 50-fold rise in the incorporation of [14C]acetate into triglyceride. The increase in lipogenic rate is exactly parallel to a coordinate rise in the activities of the key enzymes of the fatty acid biosynthetic pathway (ATP-citrate lyase, acetyl-CoA carboxylase, and fatty acid synthetase). Immunological studies indicate that the elevated acetyl-CoA carboxylase activity is the product of an increased cellular enzyme level.
3T3-L1成纤维细胞在培养过程中分化为具有脂肪细胞特征的细胞。这种转变伴随着[14C]乙酸掺入甘油三酯的量增加40至50倍。脂肪生成速率的增加与脂肪酸生物合成途径关键酶（ATP-柠檬酸裂解酶、乙酰辅酶A羧化酶和脂肪酸合成酶）活性的协同增加完全平行。免疫学研究表明，乙酰辅酶A羧化酶活性的升高是细胞内酶水平增加的产物。
