Dobrov E N, Ogareva N A, Manykin A A, Tikhonenko T I
Vopr Med Khim. 1976 Jan-Feb;22(1):98-107.
Properties of DNA in a complex with protein, which was liberated after destruction of CD phage by heat treatment in solutions with low ionic strength, were studied. DNA in the complex did not differ from free DNA under the same conditions as shown by spectra of circular dichroism and by the type of melting during the thermic denaturation. As demonstrated by viscosimetry and gradient centrifugation in cesium sulfate, 1.5% formaldehyde inhibited the dissociation of the complex studied in a medium containing 0.15 M NaCl. In the medium with 1.5% of HCHO T degrees ml of DNA in the complex was distinctly higher than T degrees ml of free DNA under the same conditions. Electron microscopy showed that the complex studied comprised DNA ;molecules, associated with protein membrane in one or several internal sites.
研究了在低离子强度溶液中通过热处理破坏CD噬菌体后释放的与蛋白质复合的DNA的性质。圆二色光谱和热变性过程中的熔解类型表明，在相同条件下，复合物中的DNA与游离DNA没有差异。粘度测定和硫酸铯梯度离心表明，1.5%的甲醛抑制了在含有0.15 M NaCl的介质中所研究复合物的解离。在含有1.5% HCHO的介质中，相同条件下复合物中DNA的Tm值明显高于游离DNA的Tm值。电子显微镜显示，所研究的复合物包含与蛋白质膜在一个或几个内部位点结合的DNA分子。
