Sato Yoshinori, Nishihara Hirofumi, Yoshida Masao, Watanabe Makiko, Rondal Jose D, Concepcion Rogelio N, Ohta Hiroyuki
Department of Bioresource Science, Ibaraki University College of Agriculture, Ami-machi, Ibaraki 300-0393, Japan.
Department of Environmental Science and Technology, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, Nagatsuda, Midori-ku, Yokohama 226-8503, Japan.
Int J Syst Evol Microbiol. 2006 May;56(Pt 5):973-978. doi: 10.1099/ijs.0.63922-0.
Taxonomic studies were performed on ten hydrogen-oxidizing, facultatively chemolithotrophic bacteria that were isolated from volcanic mudflow deposits derived from the eruption of Mt. Pinatubo in the Philippines in 1991. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these isolates belonged to the genus Cupriavidus of the Betaproteobacteria; sequence similarity values with their nearest phylogenetic neighbour, Cupriavidus basilensis, were 97.1-98.3 %. In addition to phylogenetic analysis, results of whole-cell protein profiles and biochemical tests revealed that these strains were members of two distinct species. DNA-DNA hybridizations and whole-cell protein profiles enabled these isolates to be differentiated from related Cupriavidus species with validly published names. The isolates were aerobic, Gram-negative, non-sporulating, peritrichously flagellated rods. Their G+C contents ranged from 65.2 to 65.9 mol% and their major isoprenoid quinone was ubiquinone Q-8. On the basis of these results, two novel species are proposed, Cupriavidus pinatubonensis sp. nov. [nine strains, with 1245T (=CIP 108725T=PNCM 10346T) as the type strain] and Cupriavidus laharis sp. nov. [one strain, the type strain 1263aT (=CIP 108726T=PNCM 10347T)]. It is also suggested that Ralstonia sp. LMG 1197 (=JMP 134) should be included in the species C. pinatubonensis.
对从1991年菲律宾皮纳图博火山喷发形成的火山泥流沉积物中分离出的10株氢氧化、兼性化能无机营养细菌进行了分类学研究。基于16S rRNA基因序列的系统发育分析表明，这些分离株属于β-变形菌纲的贪铜菌属；与它们最近的系统发育邻居巴兹尔贪铜菌的序列相似性值为97.1 - 98.3%。除系统发育分析外，全细胞蛋白质谱和生化测试结果表明，这些菌株属于两个不同的物种。DNA - DNA杂交和全细胞蛋白质谱使这些分离株能够与已有效发表名称的相关贪铜菌属物种区分开来。这些分离株为需氧、革兰氏阴性、不产芽孢、周生鞭毛杆菌。它们的G + C含量范围为65.2至65.9 mol%，主要类异戊二烯醌为泛醌Q - 8。基于这些结果，提出了两个新物种，皮纳图博贪铜菌新种[9株，模式菌株为1245T(=CIP 108725T=PNCM 10346T)]和拉哈尔贪铜菌新种[1株，模式菌株为1263aT(=CIP 108726T=PNCM 10347T)]。还建议将罗尔斯通氏菌属菌株LMG 1197(=JMP 134)归入皮纳图博贪铜菌物种。
