Scholten E, Lukow T, Auling G, Kroppenstedt R M, Rainey F A, Diekmann H
Institut für Mikrobiologie, Universität Hannover, Germany.
Int J Syst Bacteriol. 1999 Jul;49 Pt 3:1045-51. doi: 10.1099/00207713-49-3-1045.
A heterotrophic bacterial strain TL1T capable of aerobic denitrification was previously enriched in continuous culture from a landfill leachate treatment plant and isolated as a pure culture. The taxonomic position of this isolate within the beta-subclass of the Proteobacteria was determined by 16S rDNA sequence analysis and by conventional taxonomy including substrate spectrum, quinone type (ubiquinone Q-8) and cellular fatty acid composition. Detection of the specific polyamine 2-hydroxyputrescine supports the membership of strain TL1T in the beta-subclass of the Proteobacteria. The results of 16S rDNA sequencing showed that the strain clustered with, but was separate from, Thauera aromatica and Thauera selenatis. DNA-DNA hybridization experiments indicated that the new isolate represents a new species of the genus, for which the name Thauera mechernichensis is proposed; the type strain is DSM 12266T.
一株能够进行好氧反硝化的异养细菌菌株TL1T，先前是从垃圾渗滤液处理厂的连续培养物中富集得到，并分离为纯培养物。通过16S rDNA序列分析以及包括底物谱、醌型（泛醌Q-8）和细胞脂肪酸组成在内的传统分类学方法，确定了该分离株在变形菌纲β亚纲中的分类地位。特异性多胺2-羟基腐胺的检测支持菌株TL1T属于变形菌纲β亚纲。16S rDNA测序结果表明，该菌株与芳香陶厄氏菌和硒化陶厄氏菌聚类，但又与之分开。DNA-DNA杂交实验表明，该新分离株代表该属的一个新物种，为此提议将其命名为梅氏陶厄氏菌；模式菌株为DSM 12266T。
