Wainø M, Tindall B J, Schumann P, Ingvorsen K
Department of Microbial Ecology, University of Arhus, Denmark.
Int J Syst Bacteriol. 1999 Apr;49 Pt 2:821-31. doi: 10.1099/00207713-49-2-821.
A Gram-positive, extremely halotolerant bacterium was isolated from the Great Salt Lake, Utah, USA. The strain, designated NNT (= DSM 11805T), was strictly aerobic, rod-shaped, motile by peritrichous flagella and spore-forming. Strain NNT grew at salinities of 0-20% (w/v) NaCl. A distinctive feature of strain NNT was its optimal growth in salt-free medium. The polar lipid pattern of strain NNT consisted of phosphatidyl glycerol, diphosphatidyl glycerol and two phospholipids of unknown structure. The G + C content of its DNA was 38 mol%. The morphological, physiological and, particularly, the 16S rDNA sequence data, showed that strain NNT was associated with 'Bacillus group 1'. However, the organisms showing the greatest degree of sequence similarity to strain NNT were members of the genus Halobacillus and the species Marinococcus albus, Virgibacillus pantothenticus, Bacillus salexigens and Bacillus dipsosauri. On the basis of chemotaxonomic data, strain NNT was shown to be chemically most similar to B. salexigens and B. dipsosauri, with the greatest degree of similarity being shown to the latter organism. This was consistent with the 16S rDNA sequence data. Members of the genus Halobacillus comprise a chemically distinct group and can easily be distinguished from all other organisms of 'Bacillus group 1'. On the basis of the 16S rDNA data, chemotaxonomy and the physiology of strain NNT, it is proposed that this organism is a member of a new species, within a new genus, for which the name Gracilibacillus halotolerans is proposed. It is also proposed that B. dipsosauri be transferred to this genus as Gracilibacillus dipsosauri comb. nov. and that B. salexigens be transferred to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov. Finally, additional data is provided to support the transfer of Bacillus pantothenticus to the genus Virgibacillus, as Virgibacillus pantothenticus Heyndrickx et al. (1998).
从美国犹他州大盐湖分离出一株革兰氏阳性、极端耐盐细菌。该菌株命名为NNT（= DSM 11805T），严格需氧，杆状，周生鞭毛运动，能形成芽孢。NNT菌株在0 - 20%（w/v）NaCl盐度下生长。NNT菌株的一个显著特征是其在无盐培养基中生长最佳。NNT菌株的极性脂质模式由磷脂酰甘油、二磷脂酰甘油和两种结构未知的磷脂组成。其DNA的G + C含量为38 mol%。形态学、生理学数据，尤其是16S rDNA序列数据表明，NNT菌株与“芽孢杆菌属1组”相关。然而，与NNT菌株序列相似性最高的生物是嗜盐芽孢杆菌属成员以及白色海洋球菌、泛酸芽孢杆菌、嗜盐芽孢杆菌和迪氏芽孢杆菌。基于化学分类学数据，NNT菌株在化学上与嗜盐芽孢杆菌和迪氏芽孢杆菌最相似，与后者的相似程度最高。这与16S rDNA序列数据一致。嗜盐芽孢杆菌属成员构成一个化学性质不同的类群，可轻易与“芽孢杆菌属1组”的所有其他生物区分开来。基于NNT菌株的16S rDNA数据、化学分类学和生理学，建议将该生物归为一个新属内的新物种，为此提议命名为嗜盐纤细芽孢杆菌。还建议将迪氏芽孢杆菌转移到该属，命名为迪氏纤细芽孢杆菌新组合。并将嗜盐芽孢杆菌转移到新属盐芽孢杆菌属，命名为嗜盐盐芽孢杆菌新组合。最后，提供了更多数据以支持将泛酸芽孢杆菌转移到维氏芽孢杆菌属，即维氏泛酸芽孢杆菌Heyndrickx等人（1998年）。
